Anti-Flag tag內(nèi)參抗體

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Anti-Flag tag內(nèi)參抗體
The DYKDDDDK peptide （Flag-tag） is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant prot

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Anti-Flag tag內(nèi)參抗體

英文名稱
宿主	Mouse
別名	DDDDK Tag;DYKDDDDK Tag
應(yīng)用	WB IP IF
稀釋比例	WB 1:10000-15000. IP 1:100-300. IF 1:500-3000.
保存	Store at -20°C. Avoid freeze / thaw cycles.
儲(chǔ)存液	Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
純化方法	Affinity purification
亞型	IgG1
免疫原	A synthetic peptide coupled to KLH
性狀	液體
Public Immunogen Range	A synthetic peptide coupled to KLH
克隆號(hào)	2C5
克隆類型	Monoclonal Antibody
背景資料	The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an .. IF there is no against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an against the Flag sequence.

Anti-Flag tag內(nèi)參抗體

《High level expression of ISG12(1) promotes cell apoptosis via mitochondrial-dependent pathway and so as to hinder Newcastle disease virus replication》作者:Xiaoqin Li，Yanqing Jia，Haijin Liu，Xiangwei Wang，Zhili Chu，Ruochen Hu，Juan Ren ，Sa Xiao，Shuxia Zhang，Xinglong，Wang，Zengqi Yang 期刊:Veterinary Microbiology 影響因子:2.791 PMID:30593361

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